NISSUI 日本日水培养基 05630 Trypto-Soya Broth (SCD Broth)
| Code | Form | Package | Storage | Shelf Life |
| 05630 | Granule | 300 g | Dry, RT | 3 years |
Directlons
It is possible to isolate the bacteria from
bacteremia and septicemia.
Suspend 30.0 g of the dehydrated medium in 1,000 mL of distilled water, mix well and
heat to dissolve the medium. Distribute into appropriate containers, and sterilize by autoclaving at 121° C for 15 minutes.
Formula
The medium is commonly used for the cultivation of fastidious bacteria such as Neisseria, Brucella, streptococci and pneumococci.
As the bacteria grow rapidly on the medium,they tend to die out soon. So the medium is
not suitable for use a stock culture medium.
It is not desirable to subculture pneumococci, streptococci and Neisseria
repeatedly on this medium.For blood culture, inoculate about 5 ml of blood specimen into 50 ml of the medium.
Formula
| Componsnds | On 30.0 L |
| Peptone | 20.0 g |
| Sodlum Chlorlde | 5.0 g |
| Dextrose | 2.5g |
| Dlpotasslum Phosphate | 2.5 g |
pH 7.3土0.2