Nissui 05900 Eagle’s MEM Nissui
上海金畔生物代理日本Nissui日水品牌培养基，欢迎访问Nissui官网或者咨询我们获取更多相关产品信息。
WIth Kanamycln, Phenol Red 含卡那霉素，酚红
Wlthout L-Glutamine and Sodlum Blcarbonate, 不含L-谷氨酰胺和碳酸钠
Autoclavable 可高压灭菌
| Code | Formn | Package | Storage | Shelf Life |
| 05900 | Powder | 100 g | Cool | 1Year |
| 08160 | Powder | 9.4 g x 10 Packs | Cool | 2 Years |
Directions 使用方法：
Dissolve 9.4 g of the medium in dstilled water andadjust the volume to 1,000 mL
Sterilize by autoclaving at 121 centigrade for 15 minutes. Coolthe sterilized
medium to room temperature and adda proper amount of sterile 10% sodium bicarbonatesolution. (pH will be 7.1 – 7.4 at 37 centigrade in anatmosphere of 5% CO, in air by adding12.5- 22.0mL of sodium bicarbonate solution) This mixtureshould be stopper tightly and stored in a cool, darkplace. At the time of use, aseptically add 0.292 g ofL-glutamine (serilized) and the desired amount ofserum.
将9.4g培养基溶于蒸馏水中，将体积调整为1000 mL。
在121摄氏度下进行15分钟的高压灭菌。将灭菌培养基冷却至室温，添加适量的无菌剂10%碳酸氢钠。(加入12.5-22.0mL碳酸氢钠溶液，37℃，5%CO，空气中的pH值为7.1-7.4)。使用时，无菌添加0.292克谷氨酰胺(丝胶化)和所需的血清量。
Summary 摘要
Eagle’s MEM “Nissui” 1 is a powdered mediumprepared according to prescription published byHarry Eagle in 1959. Up to now, such media werenot autoclavable because of decomposition or
deterioration of the constituents, so that sterilizationwas performed by filtation using a membrane filter.Nissui Pharmaceutical Co., Ltd. has succeeded inpreparing an autoclavable powder medium. Thismedium is specially manufactured so that
constituents of the medium and growth of cells withthis medium are not impaired. This medium has agrowth-promoting effect for cells such as HeLa or Land other cell lines.
Storage and Expiration
Close tightly and store at 2-5C in a dark and dryplace. May be stored up to one (1) year.
紧密关闭，并在2-5℃保存在一个黑暗和干燥的地方。可储存一(1)年。
Precautions 预防措施：
Do not use the product after its expiry date. Qualityof the product is not warranted after its shelf life.When if medium or reagent touched eyes or mouth,immediately wash with plenty of water, and consulta physician.
Any medium, reagent and materials must be sterilized by autoclaving or boillng water after use,
and then disposed as industrial waste according tothe law or regulations related to dispose.
产品过期后不要使用。产品保质期后不保证质量。当介质或试剂接触眼睛或口腔时，立即用大量的水冲洗，并咨询医生。
任何介质、试剂和材料使用后必须用高压灭菌或沸水消毒，
然后按照与处置有关的法律或法规作为工业废物处理。
Formula
| Components | IN 94g/L6 |
| Sodlum Chlorlde | 6800.0 mg |
| Potasslum Chloride | 400.0 mg |
| Calclum Chlorlde | 200.0 mg |
| Magneslum Sulfate | 93.5 mg |
| Sodlum Dlhydrogon Phoaphate | 115.0 mg |
| Glucose | 1,000.0 mg |
| L-Arginlne Hydrochlorlde | 126.0 mg |
| L-Cystine Dlhydrochlordo, H2O | 31.4 mg |
| L-Tyroslne | 36.0 mg |
| L-Hstdino Hydrochlorldo, H2O | 42.0 mg |
| L-Isoleucine | 52.0 mg |
| L-Leuclne | 52.0 mg |
| L-Lyslne Hydrochlorlde | 73.0 mg |
| L-Methlonine | 15.0 mg |
| L-Phenylalanine | 32.0 mg |
| L-Threonine | 48.0 mg |
| L-Tryptophan | 10.0 mg |
| L-Vallne | 46.0 mg |
| Succinic Acld | 75.0 mg |
| Sodlum Succnato, 6H20 | 1000 mg |
| Chollne BltartrateI | 1.8 mg |
| Follc Acld | 1.0 mg |
| Inosltol | 2.0 mg |
| I Nlcotinamlde | 1.0 mg |
| Calclum Pantothenate | 1.0 mg |
| Pyrldoxal Hydrochlorlde | 1.0 mg |
| RIboflavIn | 0.1 mg |
| Thlamin Hydrochlorlde | 10 mg |
| Blotln | 0.02 mg |
| Kanamycin | 60.0 mg （Titer）) |
| Phenol Red | 6.0 mg |
图片仅供参考,请以实物为准。
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