NISSUI 05918 RPM1640培养基2 RPM1640 Medium ② 100g
NIssui日水 RPM1640培养基
| Code | Formn | Package | Storage | Shelf Life |
| 05918 | Powder | 100 g | Cool | 1 Year |
Directions
Dissolve 10.2 g of the medium in distilled waterand adjust the volume to 1,000 mL. Sterilize byautoclaving at 121 *C for 15 minutes. Cool the sterilized medium to room temperature and add aproper amount of sterile 10% sodium bicarbonate (pH will be 7.1 – 7.4 at 37*C, in an atmosphere of5% co, in air by adding 18 – 30 mL of sodiumbicarbonate solution.) Aseptically add 0.3 g Lglutamine and the desire amount or serum.
Summary
RPMI 1640 is a medium for suspension cultureinvestigated by George E. Moore, former directorof Roswell Park Memorial Institute. Up to now,such media were not autoclavable because ofdecomposition or deterioration of the constituents, so that sterilization had been performed by filtration using a membrane filter.RPMI 1640 Medium “Nissui” 2 is an autoclavable-powdered medium specially manufactured toovercome the difficulties associated with filter-sterilized media, such as contamination by Mycoplasma. This medium has growth promotingeffect to primary cultures of mouse and humanleukemia cells, various kinds of cell lines andKATO-Iil cells.
Storage and Expiration
Close tightly and store at 25*C in a dark and dryplace. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after itsshelf life.
When if medium or reagent touched eyes ormouth, immediately wash with plenty of water,and consult a physician.
Any medium, reagent and materials must be
sterilized by autoclaving or boiling water after use,and then disposed as industrial waste according tothe law or regulations related to dispose.
